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A simple sample collection using the common HPV swab is taken and the DNA is extracted. The extracted DNA undergoes bisulfite conversion before an accurate method of screening through real-time PCR analyses. The bisulfite conversion functions by converting unmethylated Cytosine nucleotides to Uracil, which is then converted to Thymine by PCR. Conversely, the methylation mark protects the methylated Cytosine nucleotides from being converted to Uracil.
